Document Type: Original Article
Department of Biology, University of Sistan and Baluchestan, Zahedan ,Iran
Department of Biology University of Sistan and Baluchestan Zahedan ,Iran
Zahedan University of Medical Sciences, Zahedan, Iran
Department of Biology University of Sistan and Baluchestan Zahedan,Iran
Background: Many studies have investigated the association between cytokine gene polymorphism and vesicoureteral reflux (VUR) susceptibility. The present study purposed to survey the association between interleukin-33 gene polymorphism (rs11792633 C/T, rs1157505C/G) and VUR.
Methods: The tetra-primer amplification mutation refractory system-polymerase chain reaction (ARMS PCR) was applied to survey two SNPs of the IL-33 gene in 100 healthy controls and 129 children with VUR disease. SPSS software was used to analyze the data.
Results: Analysis showed that one single nucleotide polymorphism (rs1157505G/C, rs11792633C/T) in the IL-33 gene is associated with VUR. This association was found between the IL-33 CG genotype and an increased risk of VUR (OR: 0.38; CI: 0.76 -0.19). The frequency of allele G at the site of the IL-33 gene (rs1157505) showed a statistical difference (0.001)between cases and controls in comparison with the wild type.
Conclusion: This is the first observation regarding IL-33 cytokine gene polymorphism and the risk of VUR. However, it is necessary to study this evidence with a larger sample size in different populations to confirm the present data.